Methods for enhancing yield of stem cell cultures and enhancing stem cell therapy
AssigneeCedars-Sinai Medical Center
InventorsEduardo Marbán, Tao-Sheng Li
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Abstract
The present application relates to methods and compositions for the generation of therapeutic cells having reduced incidence of karyotypic abnormalities. In several embodiments cardiac stem cells are cultured in an antioxidant-supplemented media that reduces levels of reactive oxygen species, but does not down regulate DNA repair mechanisms. In several embodiments, physiological oxygen concentrations are used during culture in order to increase the proliferation of stem cells, decrease the senescence of the cells, decrease genomic instability, and/or augment the functionality of such cells for cellular therapies.
Claims20
A method of increasing the yield of cardiac stem cells in culture, comprising:
- obtaining a population of cardiac stem cells isolated from a source of cardiac tissue;
- restricting oxygen concentrations in a culture environment to physiologic oxygen concentrations,
- wherein said physiologic oxygen concentrations are between about 4% and 7%;
- culturing said cardiac stem cells in said restricted oxygen culture environment;
- wherein said physiologic levels of oxygen increase the rate at which the cardiac stem cells proliferate, thereby increasing the yield of cardiac stem cells as compared to culture conditions that employ non-physiologic levels of oxygen,
- wherein the yield of cardiac stem cells is increased per unit weight of said source of cardiac tissue as compared to the yield of cardiac stem cells cultured in conditions that employ non-physiologic concentrations of oxygen, and
- wherein the per unit weight yield is increased by at least about 5% for a given time period of culturing.
A method of increasing the yield of cardiac stem cells in culture, comprising:
- obtaining a population of cardiac stem cells isolated from a source of cardiac tissue;
- restricting oxygen concentrations in a culture environment to physiologic oxygen concentrations,
- wherein said physiologic oxygen concentrations are between about 1% and 8%;
- culturing said cardiac stem cells in said restricted oxygen culture environment;
- wherein said physiologic levels of oxygen increase the rate at which the cardiac stem cells proliferate, thereby increasing the yield of cardiac stem cells as compared to culture conditions that employ non-physiologic levels of oxygen.
Dependencies are inferred from the claim text.