Methods and interfaces for single and multidimensional separations for characterization and/or identification of molecules by mass spectrometry

A method for separating molecules of interest traveling in a capture device comprising,

• a power supply to produce the electric field inside the capture device channel, a system to produce a continuous flow of electrolyte solution through the capture device;
• and a system to allow the injection of the molecules of interest into said flow stream, wherein
• molecules of interest are separated from other by the following procedures:
• an electric field of appropriate direction and magnitude is applied to the electrocapture device that captures a number of charged molecules of interest, and after that separating them by;
• decreasing the electrical field in a step wise or gradient manner, and/or increasing the applied flow rate in a step wise or gradient manner, and/or changing the electrolytic medium to a medium with a different ionic strength, pH or conductivity, where after;
• the separated molecules are ionized and injected into a mass spectrometer.