Method for production of reprogrammed cell using chromosomally unintegrated virus vector
AssigneeDNAVEC Corporation
InventorsNoemi Fusaki, Hiroshi Ban, Mamoru Hasegawa, Yoshikazu Yonemitsu
Life of this patent
Estimating the term…
Abstract
An objective of the present invention is to provide vectors for conveniently and efficiently producing ES-like cells in which foreign genes are not integrated into the chromosome. The present inventors discovered methods for producing ES-like cells from somatic cells using chromosomally non-integrating viral vectors. Since no foreign gene is integrated into the chromosome of the produced ES-like cells, they are advantageous in tests and research, and immunological rejection and ethical problems can be avoided in disease treatments.
Claims43
A method for introducing a gene into a cell to reprogram the cell, wherein the gene is introduced into the cell using a chromosomally non-integrating viral vector.
A composition for use in gene introduction for reprogramming a cell, which comprises a chromosomally non-integrating viral vector.
Use of a chromosomally non-integrating viral vector in the production of an agent for reprogramming a differentiated cell.
A chromosomally non-integrating viral vector, which carries a gene selected from the group consisting of:
- (1) the Oct gene;
- (2) the Klf gene;
- (3) the Myc gene;
- (4) the Sox gene;
- (5) the Nanog gene;
- (6) the Lin28 gene;
- (7) the SV40 Large T antigen gene;
- and
- (8) the TERT gene.
A method for producing a reprogrammed cell, which comprises the step of contacting a differentiated cell with at least one chromosomally non-integrating viral vector.
Dependencies are inferred from the claim text.