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US 2011/0287410 A1Published application
Hepatitis-c virus testing
AssigneeCommon Services Agency
InventorsPeter Simmonds, Shiu-Wan Chan, Peng Lee Yap
Life of this patent
Estimating the term…
Abstract
New styles of hepatitis C virus (HCV), referred to as HCV-3 and HCV-4, have been identified and sequenced. Antigenic regions of HCV-2, HCV-3 and HCV-4 polypeptides have been identified. Immunoassays for HCV and antibodies thereto are described, which allow more complete screening of blood samples for HCV, and allow HCV genotyping.
Claims4
A method for detecting HCV infection in a mammal, comprising:
- a) obtaining a blood sample from a mammal;
- b) isolating RNA from said blood sample;
- c) reverse transcribing said RNA into cDNA;
- d) performing PCR on said cDNA using sense and antisense primers specific for HCV, wherein said sense and antisense primers are selected from the group consisting of:
- i) SEQ ID NO:24 and SEQ ID NO:23;
- ii) SEQ ID NO:25 and SEQ ID NO:22;
- iii) SEQ ID NO:27 and SEQ ID NO:26;
- iv) SEQ ID NO:51 and SEQ ID NO:50;
- v) SEQ ID NO:52 and SEQ ID NO:53;
- vi) SEQ ID NO:42 and SEQ ID NO:40;
- vii) SEQ ID NO:42 and SEQ ID NO:41;
- viii) SEQ ID NO:43 and SEQ ID NO:40;
- and,
- ix) SEQ ID NO:43 and SEQ ID NO:41;
- and,
- e) determining whether HCV infection was detected by analyzing the results from said PCR.
13 more elements
A method for typing HCV infection in a mammal, comprising:
- a) obtaining a blood sample from a mammal;
- b) isolating RNA from said blood sample;
- c) reverse transcribing said RNA into cDNA;
- d) performing PCR on said cDNA using an antisense primer common to all HCV types and a sense primer specific for HCV type 1, 2, 3, or 4, wherein said antisense primer is selected from the group consisting of SEQ ID NO:32 and SEQ ID NO:33, and wherein said sense primer is selected from the group consisting of;
- i) SEQ ID NO:34;
- ii) SEQ ID NO:35;
- iii) SEQ ID NO:36;
- iv) SEQ ID NO:37;
- v) SEQ ID NO:38;
- and,
- vi) SEQ ID NO:39;
- and,
- e) determining what type of HCV infection was detected by analyzing the results from said PCR.
10 more elements
A method for typing HCV infection in a mammal, comprising:
- a) obtaining a blood sample from a mammal;
- b) isolating RNA from said blood sample;
- c) reverse transcribing said RNA into cDNA;
- d) performing PCR on said cDNA using a non-type-specific antisense primer and a sense primer specific for HCV types 1, 2, 3, or 4, wherein said antisense primer is selected from the group consisting of SEQ ID NO:22 and SEQ ID NO:23, and wherein said sense primer is selected from the group consisting of;
- i) SEQ ID NO:44;
- ii) SEQ ID NO:45;
- iii) SEQ ID NO:46;
- iv) SEQ ID NO:47;
- v) SEQ ID NO:48;
- and,
- vi) SEQ ID NO:49;
- and,
- e) determining what type of HCV infection was detected by analyzing the results from said PCR.
10 more elements
A method for typing HCV infection in a mammal, comprising:
- a) obtaining a blood sample from a mammal;
- b) isolating RNA from said blood sample;
- c) reverse transcribing said RNA into cDNA;
- d) performing PCR on said cDNA using an antisense primer and a sense primer specific for HCV type 3, wherein said sense and antisense primers are selected from the group consisting of:
- i) SEQ ID NO:30 and SEQ ID NO:28;
- and,
- ii) SEQ ID NO:31 and SEQ ID NO:29;
- and,
- e) determining what type of HCV infection was detected by analyzing the results from said PCR.
6 more elements
Dependencies are inferred from the claim text.