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AssigneesInstitut Pasteur Hellenique, Institut Pasteur
InventorsPenelope Mavromara, Niki Vassilaki
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The present invention relates to a novel form of core+1 protein of Hepatitis C virus (HCV), designated shorter form core+1 protein. The shorter form core+1 protein of Hepatitis C virus is the product of translation of a coding sequence consisting of all or part of a nucleotide sequence extending from nucleotide 598 to nucleotide 920 within the core+1 ORF of HCV represented on FIG. 3B. The invention also provides methods for detecting infection by Hepatitis C virus in biological samples, methods of screening compounds which interact with viral propagation in HCV infected cells or screening of compounds impaction on the expression of shorter form core+1 protein and uses of these compounds for the preparation of compositions useful for their anti-viral activities.
A shorter form core+1 protein of Hepatitis C virus (HCV) which is the product of translation of a coding sequence consisting of all or part of a nucleotide sequence extending from nucleotide 598 to nucleotide 920 within the core+1 ORF of HCV represented on FIG. 3B.
A shorter form core+1 protein of HCV obtainable in vivo by expression in transfected cells of the core+1 open reading frame (ORF) which is contained in nucleotide sequence extending from nucleotide at position 342 to nucleotide at position 920 of the nucleotide sequence represented on FIG. 3B, and which molecular weight is less than 10 kDa.
A nucleotide sequence consisting in a fragment of the nucleotide sequence extending from nucleotide 342 to nucleotide 920 represented on FIG. 3B, which fragment is capable of encoding a shorter form core+1 protein of HCV when transfected in mammalian cells under expression conditions.
A nucleotide sequence comprising a HCV core protein coding sequence, which is derived from the nucleotide sequence represented on FIG. 3B as a result of one or several mutations selected among the following:
A method of screening compounds for their capacity to interact with viral propagation in cells infected by HCV, said method comprising:
Dependencies are inferred from the claim text.