Recombinant koi herpesvirus (khv) or cyprinid herpesvirus 3 (cyhv-3) and a vaccine for the prevention of a disease caused by khv/cyhv-3 in cyprinus carpio carpio or cyprinus carpio koi
AssigneeUniversite de Liege, Faculty of Veterinary Medicine immunology-Vaccinology
InventorsBerenice Costes, Alain Francis Claude Vanderplasschen, Francois Lieffrig
Life of this patent
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Abstract
The present invention refers to a recombinant koi herpesvirus (KHV) or Cyprinid herpesvirus 3 (CyHV-3), which is immunogenic in fish, preferably in carps, more preferably in Cyprinus carpio, and to a vaccine for preventive and/or therapeutic treatment of a disease caused by koi herpesvirus (KHV) or CyHV-3. The 5 recombinant herpesvirus is used to confer immunity on fish, preferably on carps, more preferably on Cyprinus carpio, against a disease caused by koi herpesvirus (KHV) or Cyprinid herpesvirus 3 (CyHV-3).
Claims40
A recombinant koi herpesvirus Cyprinid herpesvirus 3 (CyHV-3), wherein the complete genome of said CyHV-3 virus is in the range of 290 to 300 kb.
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A DNA sequence to be used for vaccine purposes and/or prophylactic health care in fish, wherein said DNA sequence has the capability to confer immunity on fish, against a disease caused by koi herpesvirus Cyprinid herpesvirus 3 (CyHV-3).
A vector comprising a koi herpesvirus genome or a part thereof.
A method for the production of infectious particles of recombinant koi herpesvirus Cyprinid herpesvirus 3 (CyHV-3), comprising the step of introduction of any one of the following recombinant (CyHV-3) genome into permissive eukaryotic cells:
- (a) a recombinant (CyHV-3) genome comprising a bacterial artificial chromosome (BAC) vector sequence;
- or (b) a recombinant (CyHV-3) genome wherein the bacterial artificial chromosome (BAC) vector sequence is excised from the construct of (a);
- and culturing the host cell to produce recombinant (CyHV-3).
Koi herpesvirus Cyprinid herpesvirus 3 (CyHV-3) deposition number LMBP 6581 CB or a derivative thereof.
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36-39. (canceled)
Dependencies are inferred from the claim text.