Diagnosing and monitoring inflammatory diseases by measuring complement components on white blood cells
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsJoseph M. Ahearn, Susan M. Manzi, Chau-Ching Liu
Life of this patent
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Abstract
The invention is related to methods of diagnosing inflammatory diseases or conditions by determining levels of components of the complement pathway on the surface of white blood cells.
Claims20
A method for diagnosing an inflammatory disease in an individual, the method comprising,
- (a) quantitating, in a blood sample containing white blood cells from the individual, a level of a C4d and/or C3d component of the complement pathway on a surface of a T lymphocyte, B lymphocyte or monocyte in the sample, and
- (b) comparing the level in (a) with a level of C4d and/or C3d with the component of the complement pathway on the surface of a control T lymphocyte, B lymphocyte or monocyte from an individual not having the inflammatory disease, wherein an increased level of the C4d and/or C3d component of the complement pathway diagnoses the inflammatory disease in the individual.
A method for diagnosing an inflammatory disease in an individual of interest, the method comprising,
- (a) isolating lymphocytes from the individual of interest using fluorescence activated cell sorting;
- (b) determining in the lymphocytes, a level of a C4d and/or C3d component of the complement pathway on surface of the lymphocytes by determining the mean fluorescence channel, and
- (c) comparing the mean fluorescence channel in (b) with a mean fluorescence channel of lymphocytes from a control individual not having the inflammatory disease,
- wherein an increased mean fluorescence channel of the lymphocytes from the individual of interest as compared to the mean fluorescence channel of the control individual diagnoses the inflammatory disease in the individual of interest.
A computer readable medium, storing computer-executable instructions for implementing an evaluation tool using an automated system, wherein the automated system comprises memory and a processor, and wherein the evaluation tool evaluates complement component C4d deposits and/or complement component C3d deposits on surfaces of T lymphocytes, B lymphocytes or monocytes, the computer-executable instructions causing the processor to:
- receive data corresponding to complement component C4d and/or complement component C3d deposited on surfaces of T lymphocytes, B lymphocytes or monocytes,
- store the data corresponding to the complement component C4d and/or complement component C3d in the memory of the automated system;
- store the retrieved reference value in the memory of the automated system;
- compare the received data with the reference value;
- and
- store results of the comparing in the memory of the automated system.
Dependencies are inferred from the claim text.