Wuschel(wus) gene homologs
AssigneesPioneer Hi-Bred International, Inc., E. I. Du Pont de Nemours and Company
InventorsRebecca E. Cahoon, Keith S. Lowe, William J. Gordon-Kamm, Yumin Tao, Christopher J. Scelonge
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Abstract
This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
Claims15
An isolated polynucleotide comprising a nucleotide sequence encoding a WUSCHEL polypeptide containing homeodomain, wherein the polypeptide has at least 80% sequence identity over its entire length to any one of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, or 24, based on the CLUSTAL alignment method using default parameters of KTUPLE 1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, and wherein the polypeptide is capable of stimulating in vitro growth of plant tissue.
Dependencies are inferred from the claim text.