Isolation of nucleic acid
AssigneeCambridge Enterprise Limited
InventorsYii Leng Chua, Allyson Victoria Ritchie
Life of this patent
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Abstract
A method for isolating a nucleic acid comprises: binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer, washing the bound nucleic acid with a wash solution, and eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH. The wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution. Solutions, compositions, and kits for use in the methods are described.
Claims61
A method for isolating a nucleic acid, which comprises:
- (i) binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer;
- (ii) washing the bound nucleic acid with a wash solution;
- and
- (iii) eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH;
- wherein the wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution.
-40. (canceled)
A solution comprising a buffer for washing a solid phase to which a nucleic acid is bound at a first pH and eluted at a second, higher pH, wherein the pH of the solution is pH 6.0 or less.
A kit for isolation of a nucleic acid comprising:
- i) a binding buffer for binding the nucleic acid to a solid phase at a first pH;
- ii) a wash solution that comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, wherein the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the wash buffer;
- and optionally
- iii) a solution for eluting the nucleic acid from the solid phase, wherein the solution is at a second pH that is higher than the first pH.
A kit for isolation of a nucleic acid comprising:
- i) a binding buffer for binding the nucleic acid to a solid phase at a first pH;
- ii) a composition in dry form that when dissolved in a liquid provides a wash solution that comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, wherein the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution;
- and optionally
- iii) a composition in dry form that when dissolved in a liquid provides a solution for eluting the nucleic acid from the solid phase, wherein the solution is at a second pH that is higher than the first pH.
Dependencies are inferred from the claim text.