Msp-1 protein preparations from plasmodium
OwnerHermann Bujard, Rolf Lutz +3inventors
InventorsHermann Bujard, Rolf Lutz, Christian Epp, Ute Wohlbier, Christian Kauth
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Abstract
The invention relates to a merozoite surface protein 1 (“MSP-1”) preparation of Plasmodium falciparum, said MSP-1 preparation comprising a) a purified fragment p83/30 of the gp190/MSP-1 from Plasmodium without heterologous sequences, and b) a purified fragment p38/42 of the 190/MSP-1 from Plasmodium without heterologous sequences.
Claims21
A merozoite surface protein 1 (MSP-1) preparation of Plasmodium falciparum, the MSP-1 preparation comprising:
- a purified p83/30 fragment of the gp190/MSP-1 from Plasmodium falciparum without heterologous sequences, and (ii) a purified p38/42 fragment of the gp190/MSP-1 from Plasmodium falciparum without heterologous sequences.
A method for preparing a modified nucleic acid encoding a p83/30 fragment of a merozoite surface protein 1 (MSP-1) from Plasmodium falciparum, the p83/30 fragment having an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3, the method comprising the steps of:
- (a) providing an mRNA sequence encoding the p83/30 fragment of the MSP-1 from Plasmodium falciparum, the p83/30 fragment having an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3;
- (b) subjecting a region surrounding a start codon of the mRNA sequence of step (a) and containing at least one ribosomal binding site to analysis in silico to determine a secondary structure of the mRNA sequence;
- (c) identifying at least one stable hairpin-like structure in the region surrounding the start codon of step (b);
- d) Modifying (d) modifying the sequence of the mRNA in the region surrounding the start codon of step (b), wherein at least one nucleotide substitution, insertion, deletion and/or inversion destabilizes the at least one hairpin-like region, and wherein the at least one nucleotide substitution, insertion, deletion and/or inversion is effected in silico so as to encode the same amino acid sequence as is encoded by the mRNA of step (a);
- e) Subjecting (e) subjecting the at least one hairpin-like region of step (d) to analysis in silico under the same conditions as in step (b);
- (f) determining whether the at least one nucleotide substitution, insertion, deletion and/or inversion destabilizes the at least one hairpin-like region containing the at least one ribosomal binding site;
- (g) optionally repeating steps (d)-(f) until analysis in silico under the same conditions as in step (b) indicates the destabilization of the at least one hairpin-like region;
- and
- (h) preparing the modified nucleic acid.
A method of preparing composition com risin a merozoite surface .rotein 1 (MSP-1) from Plasmodium falciparum, the method comprising the steps of:
- (a) expressing a p83/30 fragment of a gp190/MSP-1 from Plasmodium falciparum without heterologous sequences in a host cell;
- (b) expressing a p38/42 fragment of a gp190/MSP-1 from Plasmodium falciparum without heterologous sequences in a host cell;
- (c) recovering the p83/30 fragment and the p38/42 fragment from the host cell;
- and
- (d) optionally combining the fragments p83/30 and p38/42 fragments.
-18. (canceled)
A method for preventing or treating malaria in a subject, the method comprising the step of:
- (a) providing a subject in need of prevention or treatment of malaria;
- and
- (b) administering to the subject a merozoite surface protein 1 (MSP-1) composition comprising:
- (i) a purified p83/30 fragment of a gp190/MSP-1 from Plasmodium falciparum, and
- (ii) a purified p38/42 fragment of a gp190/MSAP-1 from Plasmodium falciparum.
Dependencies are inferred from the claim text.