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US 2011/0257046 A1Published application
Emulsion PCR And Amplicon Capture
AssigneeApplied Biosystems, LLC
InventorBenjamin G. Schroeder
Life of this patent
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Abstract
Compositions and methods of use are disclosed for clonally amplifying target polynucleotide sequences in solution and attaching the amplicons to a surface by activation of a masked binding moiety.
Claims20
A method of attaching a polynucleotide to a surface comprising:
- placing a hydrophilic compartment of an inverse emulsion comprising a polynucleotide and a surface, wherein said polynucleotide comprises a masked binding moiety and said surface comprises a binding moiety binding partner, under conditions suitable to activate said binding moiety which binds said binding partner, whereby said polynucleotide is attached to said surface.
A method of attaching an amplicon to a bead comprising:
- (a) producing an amplicon in a hydrophilic compartment of an inverse emulsion comprising a template polynucleotide, a surface and reagents suitable for polynucleotide amplification, wherein said amplicon comprises a masked binding moiety and said surface comprises a binding moiety binding partner;
- and
- (b) uncaging said masked binding moiety under conditions in which the uncaged moiety binds said binding partner whereby said amplicon is attached to said surface.
Dependencies are inferred from the claim text.