Method for preparing high-throughput sequenceable dna from individual plaques of phages presenting peptides
AssigneeBayer Technology Services GmbH
InventorsBastian Budde, Amber Riblett, Sascha Plug, Jürgen Benting
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Abstract
The present invention relates to a process for isolating DNA from individual plaques of peptide-presenting bacteriophages in a high-throughput capacity PCR, wherein the PCR products obtained are sequenceable and a specimen of each phage studied is retained in a replicable state. The PCR is successful despite the presence of inhibitory constituents from the growth medium or the host bacteria.
Claims9
Process for the high-throughput capacity preparation of sequenceable DNA from individual plaques of peptide-presenting phages with the simultaneous retention of infectious phages, comprising at least the following steps:
- A thinning out peptide-presenting phages from a phage population on a growth medium containing host bacteria,
- B amplifying the thinned out phages by means of incubation,
- C picking the phages from the growth medium and suspending them in a medium,
- D lysing a portion of the suspended phages from step C and also using this DNA-containing lysate as a template in a polymerase chain reaction (PCR).
Dependencies are inferred from the claim text.