Nucleic acid, biomolecule and polymer identifier codes
AssigneeLife Technologies Corporation
InventorsJohn BODEAU, Heinz BREU, Kathleen PERRY, Adam HARRIS, Patrick GILLES, Miho Gilles
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AssigneeLife Technologies Corporation
InventorsJohn BODEAU, Heinz BREU, Kathleen PERRY, Adam HARRIS, Patrick GILLES, Miho Gilles
Estimating the term…
Provided herein are systems, compositions and methods for tracking, sorting and/or identifying sample polynucleotides using nucleic acid barcodes. The barcodes provided herein are oligonucleotides that are designed to be uniquely identifiable. The nucleic acid barcodes have properties that permit them to be sequenced with high accuracy and/or reduced error rates. In some embodiments, the nucleic acid barcodes are designed to have certain nucleotide sequences that make up overlapping dibase color positions (also called color positions). The order of the overlapping dibase color positions can be determined using fluorophore-encoded dibase probes in a fluorophore color calling scheme to give high fidelity reads.
A composition comprising a plurality of identifier codes
A composition comprising a plurality of identifier codes
A system comprising a plurality of individually identifiable nucleic acid barcodes comprising overlapping dibase color positions which are sequenced in a color space with at least two fluorophore encoded dibase probes in a fluorophore color calling dibase sequencing system, wherein the plurality of nucleic acid barcodes are designed to yield a color call that lacks repeating one fluorophore color that is called 4 or more times in a row.
A system comprising a plurality of individually identifiable nucleic acid barcodes comprising overlapping dibase color positions which are sequenced in a color space with at least two fluorophore encoded dibase probes in a fluorophore color calling dibase sequencing system, wherein the plurality of nucleic acid barcodes are designed to yield a color balance having the colors of the at least two fluorophore encoded dibase probes called at least once in all color positions of the barcode.
A system comprising a plurality of individually identifiable nucleic acid barcodes comprising overlapping dibase color positions which are sequenced in a color space with at least two fluorophore encoded dibase probes in a fluorophore color calling dibase sequencing system, wherein the plurality of nucleic acid barcodes are designed to yield a color call of any two nucleic acid barcodes will differ in at least three of the same color positions of both barcodes.
A system comprising a plurality of individually identifiable nucleic acid barcodes comprising overlapping dibase color positions which are sequenced in a color space with at least two fluorophore encoded dibase probes in a fluorophore color calling dibase sequencing system, wherein the plurality of nucleic acid barcodes are designed to yield a nested subset which satisfies the criterion that the plurality of the nucleic acid barcodes satisfies.
A composition comprising an individually identifiable nucleic acid barcode comprising overlapping dibase color positions which are sequenced in a color space with at least two fluorophore encoded dibase probes in a fluorophore color calling dibase sequencing system, wherein the nucleic acid barcode is designed to yield a color call that lacks repeating one fluorophore color that is called 4 or more times in a row.
A composition comprising an individually identifiable nucleic acid barcodes comprising overlapping dibase color positions which are sequenced in a color space with at least two fluorophore encoded dibase probes in a fluorophore color calling dibase sequencing system, wherein the nucleic acid barcode is designed to yield a color balance having the colors of the at least two fluorophore encoded dibase probes called at least once in all color positions of the barcode.
A composition comprising an individually identifiable nucleic acid barcode comprising overlapping dibase color positions which are sequenced in a color space with at least two fluorophore encoded dibase probes in a fluorophore color calling dibase sequencing system, wherein the nucleic acid barcode is designed to yield a color call of any two nucleic acid barcodes that differ in at least three of the same color positions of both barcodes.
A composition comprising any one individually identifiable nucleic acid barcode selected from a group consisting of SEQ ID NOS:1-96.
A composition comprising a set of individually identifiable nucleic acid barcodes selected from a group consisting of SEQ ID NOS:1-4;
A composition comprising a color position equivalent of any one individually identifiable nucleic acid barcodes selected from a group consisting of SEQ ID NOS:1-96.
A composition comprising a set of color position equivalent of individually identifiable nucleic acid barcodes selected from a group consisting of SEQ ID NOS:1-4;
Dependencies are inferred from the claim text.