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US 2011/0256641 A1Published application
Methods and Systems for Detecting Free IgE
OwnerMichael Ling, Mark Stephen Colwell +1inventors
InventorsMichael Ling, Mark Stephen Colwell, Michelle Lee Altrich
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Abstract
The present disclosure provides methods, systems, and kits for detecting IgE antibodies available to bind to the Fc epsilon receptor in a biological sample from a subject receiving anti-IgE therapy. These methods, systems, and kits find use in monitoring anti-IgE therapy and determining its efficacy.
Claims23
A method for detecting free IgE antibody in a biological sample from a patient receiving an anti-IgE therapy, the method comprising:
- a) contacting the biological sample with a capture reagent comprising at least an IgE Fc binding portion of FcεRIα polypeptide under conditions suitable for binding of free IgE antibody to the capture reagent thereby generating a free IgE antibody-capture reagent complex, wherein the capture reagent is immobilized onto a substrate via an anchoring compound that is directly or indirectly attached to the substrate;
- b) contacting the capture reagent-free IgE antibody complex with a detection reagent that binds to a region of the free IgE not bound by the capture reagent;
- and
- c) detecting the detection reagent bound to the capture reagent-free IgE antibody complex thereby detecting the presence or absence free IgE antibody in the sample.
1 more element
A system for detecting free IgE antibody, the system comprising:
- a capture reagent comprising at least an IgE Fc binding portion of FcεRIα polypeptide immobilized on a substrate via an anchoring compound that is directly or indirectly attached to the substrate;
- and
- a biological sample from a subject receiving anti-IgE therapy.
Dependencies are inferred from the claim text.