Homing endonucleases
OwnerGeorg Hausner, Jyothi Sethuraman +1inventors
InventorsGeorg Hausner, Jyothi Sethuraman, David Edgell
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Abstract
The present disclosure provides, in part, polypeptides having endonuclease activity, nucleic acid sequences for such a polypeptide, target sequences for the endonuclease, as well as vectors, cells, kits, methods, and uses of the same.
Claims40
An endonuclease comprising a polypeptide comprising the sequence set forth in SEQ ID NO:1;
- SEQ ID NO:35, an active fragment thereof, or sequence substantially identical thereto.
An endonucleases comprising a polypeptide comprising the sequence set forth in SEQ ID NO:
- 13;
- SEQ ID NO:
- 33, an active fragment thereof, or a sequence substantially identical thereto.
A polypeptide comprising one or more sequences selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 13, SEQ ID NO:
- 15, SEQ ID NO:
- 33, SEQ ID NO:
- 35, or a sequence substantially identical thereto.
A nucleic acid comprising one or more sequences selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 34, SEQ ID NO:
- 36, or a sequence substantially identical thereto.
A nucleic acid comprising one or more sequences selected from the group consisting of SEQ ID NO:
- 34, SEQ ID NO:
- 36, or a sequence substantially identical thereto.
A nucleic acid comprising a homing endonuclease recognition site comprising one or more sequences selected from the group consisting of SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:21 and SEQ ID NO:
- 22, or a sequence substantially identical thereto.
Dependencies are inferred from the claim text.