Co-metabolism of fructose and glucose in microbial production strains
AssigneeE. I. Du Pont de Nemours and Company
InventorsAndrew C. Eliot, Anthony A. Gatenby, John S. Chapman
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Abstract
Recombinant microorganisms having an improved ability to co-metabolize in medium both fructose and glucose are described. The recombinant microorganisms comprise a promoter operably linked to a native or non-native nucleotide sequence that encodes a fucose:H+ symporter polypeptide. The recombinant microorganisms are useful for the production of a variety of products including glycerol and glycerol derivatives such as 1,3-propanediol and 3-hydroxypropionic acid.
Claims9
A recombinant microorganism comprising a promoter operably linked to a native or non-native nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding a fucose:H+ symporter polypeptide, wherein the polypeptide has at least 70% amino acid identity, based on the Clustal V method of alignment, when compared to an amino acid sequence as set forth in SEQ ID NO:2, SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:30;
- (b) a nucleotide sequence encoding a fucose:H+ symporter polypeptide wherein the nucleotide sequence has at least 70% sequence identity, based on the BLASTN method of alignment, when compared to a nucleotide sequence as set forth in SEQ ID NO:1, SEQ ID NO:25, SEQ ID NO:27, or SEQ ID NO:29;
- and
- (c) a nucleotide sequence encoding a fucose:H+ symporter polypeptide, wherein the nucleotide sequence hybridizes under stringent conditions to a nucleotide sequence as set forth in SEQ ID NO:1, SEQ ID NO:25, SEQ ID NO:27, or SEQ ID NO:29;
- wherein the promoter is one that is not normally associated with the nucleotide sequence encoding a fucose:H+ symporter polypeptide and further wherein the recombinant microorganism has an improved ability to co-metabolize in medium both fructose and glucose.
Dependencies are inferred from the claim text.