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US 2011/0256593 A1Published application
Generation of Random Double-Strand Breaks in DNA Using Enzymes
AssigneeNew England Biolabs, Inc.
InventorsPei-Chung Hsieh, Chudi Guan
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Abstract
An enzyme preparation is described that includes a non-specific nuclease and a T7 Endo I mutant in a unit ratio of less than 1:200. This enzyme preparation may be used to generate double-stranded DNA fragments of a size suitable for DNA sequencing. The ends of the fragments can be readily modified as necessary to ligate adaptors or individual nucleotides to one strand of the double-stranded DNA fragments.
Claims9
A preparation, comprising:
- a non-specific nuclease and a T7 Endo I mutant in a unit ratio of less than 1:200.
Dependencies are inferred from the claim text.