Use of cyclodextrins to improve the specificity, sensitivity and yield of nucleic acid amplification reactions
AssigneeEurogentec S.A.
InventorsMarie-Claire Beckers, Philippe Cronet, Adeline Vitale, Eric Collette, Arzu Gullukaya
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Abstract
The invention is directed to methods for in vitro DNA synthesis catalysed by a DNA polymerase using cyclodextrins. The invention also relates to methods, compositions and kits comprising cyclodextrins for the amplification of a nucleic acid. The use of cyclodextrins improves the specificity, sensibility and/or yield of the amplification reaction. The invention is related more particularly to kits, compositions and methods for carrying out PCR reactions comprising a cyclodextrin.
Claims17
-16. (canceled)
A method for improving the yield, sensitivity and/or specificity of the amplification reaction of a target nucleic acid in a sample characterized in that the amplification reaction is performed in a reaction mixture comprising at least one cyclodextrin.
A method for improving the specificity and/or yield of in vitro synthesis of a nucleic acid catalyzed by a DNA polymerase comprising contacting a single stranded nucleic acid with a DNA synthesis reaction mixture comprising a DNA polymerase, a primer, dNTPs and at least one cyclodextrin.
A kit for amplification of a target nucleic acid in a sample comprising in the same container at least a cyclodextrin and at least one component selected from the group consisting of a thermostable DNA polymerase, a reaction buffer for nucleic acid amplification, dNTPs and oligonucleotide primers.
A composition for amplification of a target nucleic acid in a sample comprising a cyclodextrin, a thermostable DNA polymerase and a storage buffer.
Dependencies are inferred from the claim text.