Cell separation technique
AssigneeGrampian Biopartners Limited
InventorsChristopher Gregory, John Pound
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Abstract
In a first aspect, the present invention provides a method of removing non-viable cells from a cell population, said method comprising contacting a cell population with a compound under conditions suitable to permit binding between the compound and any non-viable cells present in the cell culture and removing at least some of the compound from said cell population. The invention further provides compositions suitable for use in these methods, further uses of such compositions and various other systems and kits.
Claims24
A method of removing non-viable cells from a cell population, said method comprising the steps of contacting a cell population with a compound under conditions suitable to permit binding between the compound and any non-viable cells present in the cell culture and removing at least some of the compound from said cell population, wherein the compound is not a proteinacious compound.
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-25. (canceled)
A method of labelling, tagging and/or identifying non-viable cells, said method comprising the steps of:
- (a) contacting a cell population with an optionally modified (i.e. tagged or labelled) dextran molecule under conditions suitable to permit binding between the dextran molecule and any non-viable cells present in the cell population;
- (b) removing unbound dextran molecule;
- and
- (c) detecting cells bound to the dextran molecule;
- wherein, cells bound to the dextran molecule comprise non-viable cells.
-29. (canceled)
An in vitro method of targeting a compound to non-viable cells, said method comprising the steps of contacting a population of cells with a dextran molecule modified to comprise one or more additional compounds to be targeted to non-viable cells, under conditions suitable to permit binding between the modified dextran molecule and any non-viable cells present in the cell population.
A kit for the removal of non-viable cells from cell populations, said kit comprising a non-protein compound, instructions for use and/or additional buffers and reagents.
Dependencies are inferred from the claim text.