Recovery and detection of microorganisms from mixed cellulose ester filtration supports by sequential treatment with methanol and acetone
AssigneeUniversite Laval
InventorsLuc Bissonnette, Andrée F. Maheux, Michel G. Bergeron, Maurice Boissinot, Jean-Luc Bernier
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Abstract
The present invention is directed to the recovery of bacteria and microparasites, particularly Cryptosporidium and Giardia, from water samples by filtration through a mixed cellulose ester membrane, partial dissolution of said membrane with methanol followed by completion in the presence of acetone, and purification and concentration using glass beads as a secondary confinement matrix.
Claims30
A method for the recovery of cells or microorganisms from a cellulose ester filtration matrix comprising or suspected of comprising the cells or microorganisms, the method comprising sequentially:
- a. Obtaining a partially disintegrated filtration matrix from the cellulose ester filtration matrix by partial dissolution of the filtration matrix in a first organic solvent and sedimentation, and;
- b. Treating the partially disintegrated filtration matrix by replacing the first organic solvent with a second organic solvent allowing complete dissolution of the partially disintegrated filtration matrix.
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A kit for the recovery of cells or microorganisms from a cellulose ester filtration matrix comprising or suspected of comprising cells or microorganisms, the kit comprising:
- a first vial comprising a first organic solvent capable of partially disintegrating the filtration matrix, a second vial comprising a second organic solvent capable of completely dissolving the filtration matrix and optionally comprising instructions for the sequential partial disintegration of the filtration matrix and complete dissolution of the filtration matrix.
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Dependencies are inferred from the claim text.