Microfluidic Continuous Flow Device

2) A microfluidic continuous flow device comprising:

• a first channel and a second channel each comprising a first and a second area wherein the first area is a compartment which is defined by partitioning elements and the second area is a space outside said compartment;
• wherein each of said channels has a first inlet to said compartment, a second inlet for introducing a cultivation medium into said space of said channel outside of said compartment, and an outlet;
• wherein each of said second inlets and each of said outlets are arranged such as to allow a flow of cultivation medium through said channel;
• wherein each of said second inlet of said first and second channel is in fluid communication with a common cultivation medium feeding line;
• and
• wherein said partitioning elements are pillars.

3) A microfluidic continuous flow device comprising:

• a first channel and a second channel each comprising a first and a second area wherein the first area is a compartment which is defined by partitioning elements and the second area is a space outside said compartment;
• wherein each of said channels has a first inlet to said compartment, a second inlet for introducing a cultivation medium into said space of said channel outside of said compartment, and an outlet;
• wherein each of said second inlets and each of said outlets are arranged such as to allow a flow of cultivation medium through said channel;
• wherein said first and said second channel are fluidly connected to each other wherein said outlet of said first channel is fluidly connected to said second inlet of said second channel;
• and
• wherein said partitioning elements are pillars.

31) A method of cultivating biological material in a microfluidic continuous flow device, wherein the method comprises:

• providing said microfluidic continuous flow device having a channel;
• wherein said channel comprises a first and a second area wherein the first area is a compartment which is defined by partitioning elements and the second area is a space outside said compartment;
• wherein through passages which are formed between said partitioning elements are dimensioned such as to retain a biological material and a sustained release composition inside said compartment;
• wherein said channel has a first inlet for introducing biological material into said compartment, a second inlet for introducing cultivation medium into said space of said channel outside of said compartment, and an outlet;
• wherein said second inlet and said outlet are arranged such as to allow a flow of cultivation medium through said channel;
• introducing a biological material and a sustained release composition into said compartment via said first inlet;
• wherein said sustained release composition releases at least one substance which supports cultivation of said biological material and which is not initially comprised in said cultivation medium;
• transporting a cultivation medium for cultivation of said biological material through said channel via said second inlet;
• and
• wherein said partitioning elements are pillars.

32) A method of cultivating biological material in a microfluidic continuous flow device, wherein the method comprises:

• providing said microfluidic continuous flow device having a first and a second channel;
• wherein each channel comprises a first and a second area wherein the first area is a compartment which is defined by partitioning elements and the second area is a space outside said compartment;
• wherein through passages which are formed between said partitioning elements are dimensioned such as to retain a biological material inside said compartment;
• wherein each channel has a first inlet for introducing biological material into said compartment, a second inlet for introducing cultivation medium into said space of said channel outside of said compartment, and an outlet;
• wherein each of said second inlets and each of said outlets are arranged such as to allow a flow of cultivation medium through said channel;
• wherein each of said second inlets of said first and second channel is in fluid communication with a common cultivation medium feeding line;
• introducing a biological material into each of said compartments via each of said respective first inlets;
• transporting a cultivation medium via said common cultivation medium feeding line through each of said two channels;
• and
• wherein said partitioning elements are pillars.

33) A method of cultivating biological material in a microfluidic continuous flow device, wherein the method comprises:

• providing said microfluidic continuous flow device having a first and a second channel;
• wherein each channel comprises a first and a second area wherein the first area is a compartment which is defined by partitioning elements and the second area is a space outside said compartment;
• wherein through passages which are formed between said partitioning elements are dimensioned such as to retain a biological material inside said compartment;
• wherein each channel has a first inlet for introducing biological material into said compartment, a second inlet for introducing cultivation medium into said space of said channel outside of said compartment, and an outlet;
• wherein each of said second inlets and each of said outlets are arranged such as to allow a flow of cultivation medium through said channel;
• wherein said first and said second channel are fluidly connected to each other wherein said outlet of said first channel is fluidly connected with said second inlet of said second channel;
• introducing biological material into each of said compartments via each of said respective first inlets;
• transporting a cultivation medium through said first and second channel by introducing a cultivation medium into said first channel via said second inlet of said first channel;
• and
• wherein said partitioning elements are pillars.