Determination of biological material ingredients
OwnerFlorian Schweigertinventor
Life of this patent
Estimating the term…
Abstract
The invention relates to a method for analysing ingredients, in particular lipids and/or vitamins and biological materials with a concentration of the biological material ingredients, to uses of relevant organic solvents or organic solvent mixtures, and to a spectrophotometer for measuring biological material ingredients. It is proposed to treat the biological materials with at least one organic solvent which extracts the ingredients, to convert the biological materials to a solidified form during the extraction, with the result that a solidified sediment and a liquid organic phase as the supernatant are formed, and to examine the extracted ingredients in the supernatant.
Claims28
A method for analyzing ingredients, especially lipids and/or vitamins and biological materials with an enrichment of the ingredients from the biological materials, in which the biological materials are treated with at least one organic solvent which extracts the ingredients, with the biological materials being converted in the extraction into a consolidated form so that a consolidated sediment and a supernatant of liquid organic phase are formed, and the ingredients extracted into the supernatant are investigated.
The method as claimed in any of the preceding claims, characterized in that tissues, organs and/or body fluids are used as biological materials.
The method as claimed in any of the preceding claims, characterized in that polarly protic solvents, in particular alcohols, are used as organic solvent.
The method as claimed in any of the preceding claims, characterized in that the solvents are employed in the form of solvent mixtures, in particular in the form of solvent mixtures which comprise polar and nonpolar solvents.
The method as claimed in any of the preceding claims, characterized in that surfactants, especially nonionic surfactants, are also employed.
The method as claimed in any of the preceding claims, characterized in that the biological materials are treated with the organic solvents in a ratio of 1:50, in particular in a ratio of 1:10, preferably in a ratio of 1:3.
The method as claimed in any of the preceding claims, characterized in that the method is carried out at a temperature in the range from 5° C. to 60° C., in particular in the range from 10° C. to 40° C.
The method as claimed in any of the preceding claims, characterized in that the method is carried out under a pressure, between 0.5 bar to 5 bar, in particular between 0.8 bar to 2 bar.
The method as claimed in any of the preceding claims, characterized in that the biological materials are treated for extracting the ingredients for a period of from 10 seconds to 10 minutes, in particular from 10 seconds to 5 minutes, preferably from 10 seconds to 3 minutes.
The method as claimed in any of the preceding claims, characterized in that the ingredients are converted into a lipophilic form and/or lipophilically modified before the extraction.
The method as claimed in any of the preceding claims, characterized in that water-soluble ingredients are detected by extracting lipophilically or lipophilically modified products which have been obtained from enzyme-substrate reactions.
The method as claimed in any of the preceding claims, characterized in that at least one of the ingredients comprises carbohydrates, lipids, nucleic acids, proteins, macroelements, trace elements and/or vitamins.
The method as claimed in any of the preceding claims, characterized in that the ingredients are investigated by spectrometry, in particular colorimetry, preferably fluorimetry.
The use of organic solvents or organic solvent mixtures for analyzing ingredients of biological materials, where the analysis is employed for the diagnosis and/or monitoring of therapy of disorders, especially of metabolic disorders and/or deficiency disorders.
A spectrophotometer, in particular hand photometer, for measuring ingredients of biological materials in a cylindrical vessel, characterized in that the ray path of the photometer is adjusted so that the measurement covers an upper half of the vessel.
Dependencies are inferred from the claim text.