Methods
OwnerR. Jeremy Nichols, Dario Alessi +1inventors
InventorsR. Jeremy Nichols, Dario Alessi, Nicolas Dzamko
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Abstract
A method for assessing the effect of a test compound on LRRK2 in a cell-based system, the method comprising the steps of a) assessing the effect of exposing the cell-based system comprising LRRK2 to the test compound on the phosphorylation state of Ser910 and/or Ser935 of the LRRK2; and/or b) assessing the effect of exposing the cell-based system comprising LRRK2 to the test compound on the binding of the LRRK2 to a 14-3-3 polypeptide. The method is considered to be useful in assessing the effect of putative LRRK2 inhibitors in cell based systems, including in vivo systems.
Claims17
A method for assessing the effect of a test compound on LRRK2 in a cell-based system, the method comprising the steps of
- a) assessing the effect of exposing the cell-based system comprising LRRK2 to the test compound on the phosphorylation state of Ser910 and/or Ser935 of the LRRK2;
- and/or
- b) assessing the effect of exposing the cell-based system comprising LRRK2 to the test compound on the binding of the LRRK2 to a 14-3-3 polypeptide.
An antibody that binds specifically to LRRK2 phosphorylated at Ser910;
- or an antibody that binds specifically to LRRK2 phosphorylated at Ser935;
- or an antibody that binds specifically to LRRK2 that is not phosphorylated at Ser910;
- or an antibody that binds specifically to LRRK2 that is not phosphorylated at Ser935.
A kit of parts comprising two or more of 1) an antibody that binds specifically to LRRK2 phosphorylated at Ser910 or an antibody that binds specifically to LRRK2 that is not phosphorylated at Ser910 2) an antibody that binds specifically to LRRK2 phosphorylated at Ser935 an antibody that binds specifically to LRRK2 that is not phosphorylated at Ser935 and 3) a 14-3-3 polypeptide or an antibody that specifically binds to a 14-3-3 polypeptide.
Use of 1) an antibody that binds specifically to LRRK2 phosphorylated at Ser910 or an antibody that binds specifically to LRRK2 that is not phosphorylated at Ser910 2) an antibody that binds specifically to LRRK2 phosphorylated at Ser935 or an antibody that binds specifically to LRRK2 that is not phosphorylated at Ser935 and/or 3) a 14-3-3 polypeptide or an antibody that specifically binds to a 14-3-3 polypeptide in a method for assessing the effect of a test compound on LRRK2 in a cell-based system.
A purified preparation or kit of parts comprising an LRRK2 polypeptide or polynucleotide or antibody binding specifically to LRRK2;
- and a 14-3-3-polypeptide or polynucleotide or antibody binding specifically to a 14-3-3 polypeptide.
Dependencies are inferred from the claim text.