Molecules interacting with casl (mical) polynucleotides, polypeptides, and methods of using the same
OwnerAlex L. Kolodkin, Jon R. Terman +3inventors
InventorsAlex L. Kolodkin, Jon R. Terman, Tiany Mao, Ronald J. Pasterkamp, Hung-Hsiang Yu
Life of this patent
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Abstract
The present invention provides MICAL and MICAL-Like polypeptides and polynucleotides. Also provided are methods that for identifying agents that affect axon growth and placement. Furthermore, provided herein are methods for affecting axon growth and placement.
Claims83
An isolated polypeptide comprising an N-terminal MICAL domain, a calponin homology domain, a LIM domain, a proline rich region, and a plexin interacting region, wherein the polypeptide has monooxygenase activity.
An isolated polypeptide comprising a plexin interacting region at least 90% identical to a plexin interacting region of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, or SEQ ID NO:12, wherein the polypeptide has plexin interacting activity.
An isolated polypeptide comprising an N-terminal MICAL domain of Drosophila MICAL 1, or the N-terminal MICAL domain of human MICAL 1, 2, or 3, or a conservative variant thereof.
An isolated polypeptide comprising a calponin homology domain at least 90% identical to the calponin homology domain of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, or SEQ ID NO:12, and wherein the polypeptide is involved in actin filament binding.
An isolated polypeptide comprising a LIM domain at least 90% identical to the LIM domain of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, or SEQ ID NO:12, and wherein the polypeptide specifically interacts with a LIM-binding protein.
An isolated polypeptide comprising a proline rich region at least 90% identical to the proline rich region of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, or SEQ ID NO:12, and wherein the polypeptide interacts with a polypeptide comprising an SH3-domain.
35-39. (canceled)
A double-stranded RNA molecule comprising a first RNA strand that specifically hybridizes to an mRNA encoding a MICAL polypeptide and a second RNA strand that is the reverse complement of said first strand, wherein said double-stranded RNA molecule is at least 15 base pairs in length.
An isolated polypeptide or a functional peptide portion thereof, comprising a calponin homology domain, a LIM domain, a proline rich region, and a plexin interacting region, and having plexin-interacting activity.
87-153. (canceled)
Dependencies are inferred from the claim text.