Compositions for use in identification of bacteria
OwnerDavid J. Eckerinventor
Life of this patent
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Abstract
The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis.
Claims42
A kit comprising an oligonucleotide primer pair comprising a forward primer and a reverse primer, each comprising between 13 and 35 linked nucleotides in length, wherein:
- the forward primer comprises at least 70% sequence identity with SEQ ID NO.:
- 1465 and the reverse primer comprises at least 70% sequence identity with SEQ ID NO.:
- 1466,
- the forward primer comprises at least 70% sequence identity with SEQ ID NO.:
- 1467 and the reverse primer comprises at least 70% sequence identity with SEQ ID NO.:
- 1468, or
- the forward primer comprises at least 70% sequence identity with SEQ ID NO.:
- 1469 and the reverse primer comprises at least 70% sequence identity with SEQ ID NO.:
- 1470.
An oligonucleotide primer pair comprising a forward primer and a reverse primer, each comprising between 13 and 35 linked nucleotides in length, wherein the forward primer comprises at least 70% sequence identity with SEQ ID NO.:
- 1465, SEQ ID NO.:
- 1467, or SEQ ID NO.:
- 1469.
An oligonucleotide primer pair comprising a forward primer and a reverse primer, each comprising between 13 and 35 linked nucleotides in length, wherein the reverse primer comprises at least 70% sequence identity with SEQ ID NO.:
- 1466, SEQ ID NO.:
- 1468, or SEQ ID NO.:
- 1470.
A method for identifying a Staphylococcus aureus bioagent in a sample comprising:
- a) amplifying a nucleic acid from said sample using an oligonucleotide primer pair comprising a forward primer and a reverse primer, each comprising between 13 and 35 linked nucleotides in length, said forward primer comprising at least 70% sequence identity with SEQ ID NO.:
- 1465, SEQ ID NO.:
- 1467, or SEQ ID NO.:
- 1469 and said reverse primer comprising at least 70% sequence identity with SEQ ID NO.:
- 1466, SEQ ID NO.:
- 1468, or SEQ ID NO.:
- 1470, wherein said amplifying generates at least one amplification product that comprises between 45 and 200 linked nucleotides;
- and
- b) determining the molecular mass of said at least one amplification product by mass spectrometry.
Dependencies are inferred from the claim text.