Products and methods for tissue preservation
AssigneeGenVault Corporation
InventorMichael Hogan
Life of this patent
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Abstract
The present application relates to methods for increasing stability of formalin fixed, optionally paraffin embedded biological material. In one example, DNAse inhibitors and/or RNAse inhibitors are combined with the biological material or formalin at the time of fixation or shortly prior.
Claims26
A composition comprising:
- a. a degradation inhibitor, and
- b. formalin.
(canceled)
-12. (canceled)
-16. (canceled)
A composition comprising:
- a. a small molecule inhibitor of the phosphoryl transferase superfamily, and
- b. a reactive oxygen species scavenger.
-20. (canceled)
A method for preserving a polynucleotide in a biologic sample comprising:
- a. contacting the biologic sample with formalin,
- b. contacting the biologic sample with a degradation inhibitor.
A method for analyzing a polynucleotide comprising
- a. obtaining a biologic sample, wherein the sample has been contacted with formalin solution, contacted with a degradation inhibitor, dehydrated, embedded, and stored;
- b. purifying the polynucleotide, and
- c. analyzing the polynucleotide.
-45. (canceled)
A formalin fixed paraffin embedded tissue containing a plurality of polynucleotides wherein at least 5% of the polynucleotides strands are greater than 300 base pairs.
A polynucleotide recovered from fixed tissue:
- a. wherein the polynucleotide strand is at least 300 base pairs,
- b. wherein the tissue was fixed in formalin,
- c. wherein the formalin comprises a degradation inhibitor.
- d. wherein the fixed tissue was embedded in paraffin.
-53. (canceled)
A polynucleotide recovered from fixed tissue:
- a. wherein the polynucleotide strand is at least 300 base pairs,
- b. wherein the tissue was fixed in formalin,
- c. wherein the tissue was washed in a ethanol-water wash comprising a water-soluble reactive oxygen species scavenger, and
- d. wherein the fixed tissue was embedded in paraffin.
Dependencies are inferred from the claim text.