Nanocrystals with functional ligands
AssigneeLife Technologies Corporation
InventorsEric Tulsky, Eric Welch, Bruce Branchaud, Roman Rozhkov
Life of this patent
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Abstract
Coated fluorescent semiconductor nanoparticles having an organic surface layer of multi-functional surface ligands that include a nanocrystal binding center and one or more covalently attached functional groups or reactive functional groups are described as well as water-dispersible nanoparticles having an organic surface layer or multi-functional surface ligands and methods for the preparation and use of such coated nanoparticles.
Claims42
A method for preparing a nanoparticle comprising:
- admixing one or more first nanocrystal precursors and one or more multi-functional ligands of general Formula I:
- (X)n-(L-R)y I
- wherein:
- each X is independently a nanocrystal binding center selected from phosphonic acid, phosphinic acid, phosphine, phosphine oxide, carboxylic acid, amine, imidazole, thiol, mercaptan, sulfonate, and selenate;
- each L is independently a linker selected from a covalent bond, saturated or unsaturated aliphatic chain of from 2 to about 500 carbon atoms, polyethylene glycol having from 2 to about 500 carbon atoms, or polyester having from 2 to about 100 carbon atoms;
- each R independently is hydrogen or a functional group selected from halide, hydroxyl, carboxylic acid, carboxylic acid ester, ester, succinimidyl ester, alkyl ester, benzyl ester, aldehyde, amine including primary, secondary, or tertiary amine, polyamine, thiol, isocyanate, cycloalkyl, heterocycloalkyl, benzyl, aryl, heteroaryl, alkaryl, heterocycle, imidazole, pyridone, tetrazole, cyano, cyanoalkyl, thiol, thioalkyl, alkoxy, thioalkoxy, ether, alkyl ether, thioether, polyether, carbamates, azide, silyl, silyl ester, a polyethylene glycol moiety having from 2 to about 500 carbon atoms, a polyester having from 2 to about 20 carbon atoms, and combinations thereof;
- and
- y and n are independently 1 to 3 with the proviso that when y is 1, R is not hydrogen and when y is 2 or 3, at least one R is not hydrogen;
- in a solvent to form a mixture;
- and
- heating the mixture.
A nanoparticle having a coating, said coating comprising one or more multi-functional ligands of Formula I:
- (X)n-(L-R)y I
- wherein:
- each X is independently a nanocrystal binding center selected from phosphonic acid, phosphinic acid, phosphine, phosphine oxide, carboxylic acid, amine, imidazole, thiol, mercaptan, sulfonate, and selenate;
- each L is independently a linker selected from a covalent bond, saturated or unsaturated aliphatic chain of from 2 to about 500 carbon atoms, polyethylene glycol having from 2 to about 500 carbon atoms, or polyester having from 2 to about 100 carbon atoms;
- each R independently is hydrogen or a functional group selected from halide, hydroxyl, carboxylic acid, carboxylic acid ester, ester, succinimidyl ester, alkyl ester, benzyl ester, aldehyde, amine including primary, secondary, or tertiary amine, polyamine, thiol, isocyanate, cycloalkyl, heterocycloalkyl, benzyl, aryl, heteroaryl, alkaryl, heterocycle, imidazole, pyridone, tetrazole, cyano, cyanoalkyl, thiol, thioalkyl, alkoxy, thioalkoxy, ether, alkyl ether, thioether, polyether, carbamates, azide, silyl, silyl ester, a and combinations thereof;
- and
- y and n are independently 1 to 3 with the proviso that when y is 1, R is not hydrogen and when y is 2 or 3, at least one R is not hydrogen.
Dependencies are inferred from the claim text.