Antioxidant Compositions and Methods Thereto

A method for measuring antioxidant activity of a test sample having water and lipid soluble ingredients by measuring oxygen uptake, comprising:

• combining an oxidizable target molecule and the test sample in a solvent of acetone/water/detergent to form a test mixture at 37° C. under conditions where oxygen is present in equilibrium with air;
• adding an azo radical initiator to the test mixture;
• and
• measuring area under an oxygen uptake curve of the test mixture to provide a measure of the antioxidant activity of the test sample.

A method of measuring stability of an antioxidant composition over time, the composition having water and lipid soluble ingredients, by measuring oxygen uptake of the composition at a first time point and at a second, later, time point, comprising:

• combining an oxidizable target molecule and the antioxidant composition in a solvent of acetone/water/detergent to form a test mixture at 37° C. under conditions where oxygen is present in equilibrium with air;
• adding an azo radical initiator to the test mixture;
• and
• measuring area under an oxygen uptake curve of the test mixture to provide a measure of the antioxidant activity of the antioxidant composition at the first time point and at the second time point;
• wherein a difference between the area under the oxygen uptake curve for the first time point and the area under the oxygen uptake curve for the second time point is a measure of stability of the antioxidant composition over time.