Novel use of an il-12 receptor splice variant and molecular assay to quantify expression thereof
AssigneeTrudeau Institute
InventorsRichard T. Robinson, Andrea M. Cooper
Life of this patent
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Abstract
The present invention describes compositions for both diagnostic and therapeutic applications. In one embodiment, the present invention contemplates a vaccine formulation comprising an antigen and IL12Rβ1 isoform 2. In some embodiments, this invention relates to a method of quantifying the ratio of IL12Rβ1 transcript and a splice variant thereof in a sample, including but not limited to at the cDNA level. In other embodiments, this invention relates to a method of augmenting an immune response by administering, inhibiting and/or inducing IL12Rβ1 isoform 2.
Claims29
A vaccine formulation comprising an antigen and the IL12Rβ1 isoform 2.
A method for detecting a transcript and a splice variant thereof, comprising:
- a) providing:
- i) a sample comprising cDNA molecules corresponding to the transcript encoding IL12Rβ1 isoform 1 and the splice variant encoding IL12Rβ1 isoform 2, at least one of said cDNA molecules comprising a transmembrane-encoding region,
- ii) a PCR primer set flanking said transmembrane-encoding region of said cDNA molecules, and
- b) amplifying said cDNA with said PCR primer set so as to produce PCR products, and
- c) detecting the PCR products corresponding to the cDNA molecules of said transcript encoding IL12Rβ1 isoform 1 and said splice variant encoding IL12Rβ1 isoform 2.
A method of augmenting an immune response, comprising:
- a) providing:
- i) a subject, and
- ii) a composition comprising the IL12Rβ1 isoform 2, and
- b) administering the isoform to said subject.
A primer having the nucleotide sequence of SEQ ID NO:1.
A method of augmenting an immune response, comprising:
- a) providing:
- i) a subject, and
- ii) a compound capable of inducing expression of a peptide isoform of an IL12Rβ1 splice variant, and
- b) administering said compound to said subject such that expression of said peptide isoform is induced.
Dependencies are inferred from the claim text.