Chondrogenic progenitor cells, protocol for derivation of cells and uses thereof
AssigneeThe University Court of the University of Edinburgh
InventorsBrendon Stewart Noble, David Matthew Pier
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Abstract
The present invention provides an isolated population of chondrocyte precursor cells wherein 1% or less of the cells express Oct4, Nanog and/or TRA-1-60, 7% or less of the cells express no collagen II, collagen X, CD105 or Stro-1 and 85% or more of the cells express CBFA1, methods for preparing such cells and uses of chondrocyte cells derived from said precursor cells.
Claims10
1-10. (canceled)
An isolated population of chondrocyte precursor cells which are characterized by the loss of transdifferentiation potential.
A method for the preparation of chondrocyte precursor cells, comprising the steps of:
- (a) differentiating a population of primate pluripotent stem (pPS) cells in chondrogenic media until the cells are greater than 75% confluent;
- (b) washing the cells and resuspending in a defined minimal growth medium;
- and
- (c) culturing the cells obtained in (b) for a further period until the cells are differentiated into a population of chondrocyte precursor cells which are characterized by the loss of transdifferentiation potential.
A method of administering a cellular composition comprising a chondrocyte lineage cell to a subject such that the cellular composition engrafts in the subject without generating an immune response to the cellular composition that would reject the engrafted cellular composition comprising 1) obtaining a cellular composition comprising a chondrocyte lineage cell and 2) administering the chondrocyte lineage cell to the subject without administering an immuno-modulatory compound to the subject.
Dependencies are inferred from the claim text.