Method and device for selectively targeting cells within a three -dimensional specimen
AssigneeCyntellect, Inc.
InventorsTimothy M. Eisfeld, Manfred R. Koller, Bernhard O. Palsson
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Abstract
The invention provides an apparatus for electromagnetically affecting a particle of interest in a specimen. The apparatus includes (a) a stage capable of supporting the specimen; (b) a detector including at least one camera, wherein the detector is capable of resolving a particle of interest within the specimen; (c) a means for locating the particle of interest in three dimensions; (d) a means for focusing electromagnetic radiation to a focal volume within the specimen; and (e) a means for adjusting the relative positions of the stage and electromagnetic radiation focusing means, thereby positioning the particle of interest within the focal volume.
Claims3
An apparatus for electromagnetically affecting a particle of interest in a specimen, comprising:
- (a) a stage capable of supporting the specimen;
- (b) a detector comprising at least one camera, wherein the detector is capable of resolving a particle of interest within the specimen;
- (c) a means for locating the particle of interest in three dimensions;
- (d) a means for focusing electromagnetic radiation to a focal volume within the specimen;
- and
- (e) a means for adjusting the relative positions of the stage and electromagnetic radiation focusing means, thereby positioning the particle of interest within the focal volume.
A method for electromagnetically affecting a particle of interest in a specimen, comprising:
- (a) storing a plurality of two-dimensional sectional representations in a computer memory, wherein each representation corresponds to one of the focal planar regions, wherein a particle of interest can be discerned in at least one of the two-dimensional sectional representations;
- (b) producing a three-dimensional representation of at least a portion of the specimen from the plurality of two-dimensional sectional representations;
- (c) locating the particle of interest in the specimen based on the three-dimensional representation of at least a portion of the specimen;
- and
- (d) focusing electromagnetic radiation to a focal volume, the focal volume intersecting a portion of the specimen containing the particle of interest.
A method for selectively targeting specific particles in a specimen, comprising:
- focusing electromagnetic radiation to a focal volume with a specimen, the focal volume intersecting a portion of the specimen containing a particle of interest, wherein the radiation within the focal volume substantially affects only the portion of the specimen within the focal volume, the portion of the specimen within the focal volume being surrounded by an envelope of substantially unaffected specimen.
Dependencies are inferred from the claim text.