Bio-analysis using ball-ended incident and output optical fibers
AssigneeBioptic, Inc.
InventorsVarouj D. AMIRKHANIAN, Shou-Kuan TSAI
Life of this patent
Estimating the term…
Abstract
A detection optics configuration for bio-analysis, in which the direction of incident radiation, the axis of the separation channel, and the direction of collection of the output radiation are coplanar at the detection zone. The detection configuration incorporates ball-end optical fibers to direct incident radiation at and collection of output radiation from the detection zone. The detection optics configuration may be implemented in an improved bio-separation instrument, in particular a capillary electrophoresis instrument.
Claims20
A detection system for a bio-separation device in which bio-separation of a sample takes place, comprising:
- a separation channel having a first longitudinal axis along which the sample undergoes separation into sample components, and a detection zone defined along the separation channel through which sample components pass;
- a radiation source;
- an detector;
- a incident light guide having a second longitudinal axis, directing incident radiation from the radiation source to the detection zone, causing radiation to be emitted by sample components as they pass through the detection zone;
- and
- an emission light guide having a third longitudinal axis, collecting and directing emitted radiation from the detection zone to the detector,
- wherein the emission light guide and the incident light guide are positioned on opposite sides of the separation channel, and wherein the first, second and third longitudinal axis are substantially coplanar at least at or near the detection zone.
A detection system for a bio-separation device in which bio-separation of a sample takes place, comprising:
- a separation channel having a first longitudinal axis along which the sample undergoes separation into sample components, and a detection zone defined along the separation channel through which sample components pass;
- a radiation source;
- a detector;
- an incident light guide having a second longitudinal axis, directing incident radiation from the radiation source to the detection zone, causing radiation to be emitted by sample components as they pass through the detection zone;
- and
- an emission light guide having a third longitudinal axis, collecting and directing emitted radiation from the detection zone to the detector,
- wherein the emission light guide and the incident light guide are positioned on opposite sides of the separation channel, and at least one of the incident light guide and emission light guide comprises an optical fiber.
A cartridge for bio-separation of a sample, comprising:
- a body,
- a separation channel defined in the body, having a first longitudinal axis along which the sample undergoes separation into sample components, and a detection zone defined along the separation channel through which sample components pass;
- a incident light guide comprising a first optical fiber having a terminating integral ball-end structure, directing incident radiation from an external radiation source to the detection zone, causing radiation to be emitted by sample components as they pass through the detection zone;
- and
- an emission light guide comprising a second optical fiber having a terminating integral ball-end structure, collecting and directing emitted radiation from the detection zone to an external detector.
A method of detecting bio-separation of a sample, comprising:
- providing a separation channel having a first longitudinal axis along which the sample undergoes separation into sample components, and defining a detection zone defined along the separation channel through which sample components pass;
- providing a radiation source;
- providing an detector;
- providing a incident light guide having a second longitudinal axis, directing incident radiation from the radiation source to the detection zone, causing radiation to be emitted by sample components as they pass through the detection zone;
- and
- providing an emission light guide having a third longitudinal axis, collecting and directing emitted radiation from the detection zone to the detector,
- wherein the emission light guide and the incident light guide are positioned on opposite sides of the separation channel, and wherein the first, second and third longitudinal axis are substantially coplanar at least at or near the detection zone.
Dependencies are inferred from the claim text.