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US 2011/0252873 A1Published application
Dynamic thermal focusing of chromatographic separations
AssigneeProxeon Biosystems A/S
InventorOle Vorm
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Abstract
There is provided a method for thermally focusing the elution times of compounds of a liquid chromatography system comprising a pre-column and a separation column as well as to provide a liquid chromatography system, wherein the chromatographic separation of a mixture of substances is optimized.
Claims10
A method for thermally focusing an analyte passing through a pre-column and a separation column for liquid chromatography, which method comprises the steps:
- operation of the pre-column, during an enrichment phase for enriching a sample in the pre-column, at a first temperature that is lower than ambient temperature and the operating temperature of the separation column and lower than 15° C.;
- heating the pre-column, after completion of the enrichment phase, to a second temperature that is higher than the operating temperature of the separation column and higher than 20° C.;
- eluting the analyte contained in the pre-column to the separation column;
- cooling of the sample to the operating temperature of the separation column;
- eluting the analyte contained in the separation column;
- and
- thermally desorbing non-eluted sample from the separation column by increasing the temperature to a temperature that is higher than the second temperature of the pre-column;
- wherein the ID of the pre-column is from 150% to 750% of the ID of the separating column.
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Dependencies are inferred from the claim text.