Non-Fluorescent Quencher Compounds and Biomolecular Assays
AssigneeLife Technologies Corporation
InventorsGregory J. Ewing, Deborah Kay Fieri, Khairuzzaman Bashar Mullah, Ronald J. Graham
Life of this patent
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Abstract
Bis-diazo, triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides and polypeptides. The quencher moieties are non-fluorescent and accept energy transfer from fluorescent reporter labels by any energy-transfer mechanism, such as FRET. Fluorescence quencher compositions are useful in preparing quencher labeled biomolecules for various molecular biology assays based on fluorescence detection.
Claims20
1-54. (canceled)
A compound comprising:
- a quencher moiety comprising structure (Q1) linked to a reactive linking group, wherein Q1 comprises the following structure:
- wherein:
- R1, R2, R4, R5, R6, R7, R8, R9, R10, R11, R12, R13 and R14 are each, independently of one another, selected from hydrogen, an electron-withdrawing group and an electron-donating group, or, alternatively R4 may be taken together with R5 or R6 may be taken together with R7 to form a benzo group,
- wherein:
- (i) at least one of R1, R2, R4, R5, R6, R7, R8, R9, R10, R11, R12, R13 or R14 is an electron-withdrawing group;
- and (ii) at least one of R1, R2, R4, R5, R6, R7, R8, R9, R10, R11, R12, R13 or R14 is an electron-donating group.
Dependencies are inferred from the claim text.