Soybean Seed and Oil Compositions and Methods of Making Same

A method of producing soybean seed comprising a linolenic acid content of 0.5% to 6% of total seed fatty acids by weight and an oleic acid content of 55% to 80% of total seed fatty acids by weight, comprising the steps of:

• a) growing one or more soybean plants that comprise a transgene that decreases the expression of an endogenous soybean FAD2-1 gene and at least one loss-of-function mutation in an endogenous soybean FAD3 gene, wherein said soybean plants yield seed having a seed fatty acid composition comprising a linolenic acid content of 0.5% to 6% of total seed fatty acids by weight and an oleic acid content of 55% to 80% of total seed fatty acids by weight, and,
• b) harvesting seed from said plant.

A soybean seed, said seed having a fatty acid composition comprising a linolenic acid content of 0.5% to 6% of total seed fatty acids by weight and an oleic acid content of 55% to 80% of total seed fatty acids by weight, a transgene that decreases the expression of an endogenous soybean FAD2-1 gene, and at least one loss-of-function mutation in an endogenous soybean FAD3 gene.

A method of producing soybean seed comprising a linolenic acid content of about 0.5% to 6% of total seed fatty acids by weight, a saturated fatty acid content of about 2% to 8% by weight and an oleic acid content of 55% to 80% of total seed fatty acids by weight, comprising the steps of:

• a) growing one or more soybean plants that comprise at least one transgene that decreases the expression of both an endogenous soybean FAD2-1 and an endogenous FATB gene, and at least one loss-of-function mutation in an endogenous soybean FAD3 gene, wherein said soybean plants yield seed having a seed fatty acid composition comprising a linolenic acid content of about 0.5% to 6% of total seed fatty acids by weight, a saturated fatty acid content of about 2% to 8% by weight, and an oleic acid content of 55% to 80% of total seed fatty acids by weight;
• and,
• b) harvesting seed from said plant.

A soybean seed, said seed having a fatty acid composition comprising a linolenic acid content of about 0.5% to 6% of total seed fatty acids by weight, a saturated fatty acid content of about 2% to 8% by weight and an oleic acid content of 55% to 80% of total seed fatty acids by weight, at least one transgene that decreases the expression of both an endogenous soybean FAD2-1 and an endogenous FATB gene, and at least one loss-of-function mutation in an endogenous soybean FAD3 gene.