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US 2011/0239332 A1Published application
Propagation of switchgrass and miscanthus
OwnerRonald E. Rothrock, JR.inventor
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Abstract
Methods and materials for clonally increasing switchgrass and Miscanthus shoot multiplication rate in culture media are disclosed. Clonal multiplication may be carried out at rates acceptable for various commercial applications.
Claims27
A method of multiplying switchgrass shoots, the method comprising growing a plurality of switchgrass shoots at about 31° C. in a multiplication media comprising Cu++ at a concentration of from about 0.040 μM to about 45 μM.
A method for clonally propagating Miscanthus under conditions of 50-100 μE of light, 16 hours of light per day and 31° C., comprising:
- a) growing stem sections comprising at least a portion of a node in an semi-solid establishment media at pH 5.6 with 6 g/L agar or a liquid establishment media at pH 3.3 to form shoots, said establishment media comprising MS plus vitamins, 30 g/L sucrose (87.7 mM), 3.7 micro-Molar MgCl2 (750 mg/L of MgCl2.6H2O), 5 mg/L BAP (22.2 micro-Molar), and 0.25 mg/L NAA (1.3 micro-Molar);
- b) growing shoots formed in step a) in a semi-solid multiplication media at pH 5.7 with 6 g/L agar or a liquid multiplication media at pH 3.0 to multiply said shoots, said multiplication media comprising MS plus vitamins, 3% sucrose, 5.0 mg/L BAP, 0.25 mg/L TDZ, and 1.0 mg/L PBZ;
- and
- c) growing shoots formed in step b) in a rooting media comprising MS plus vitamins, 3% sucrose, and 10.0 mg/L NAA with 6 g/L agar at pH 5.7 to form Miscanthus plantlets.
1 more element
Dependencies are inferred from the claim text.