Isolation and use of fad2 and fae1 from camelina
AssigneeTargeted Growth, Inc.
InventorsCarolyn HUTCHEON, Renata F. Ditt, Christine K. Shewmaker
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Abstract
The present invention provides isolated FAD2 and FAE1 genes and FAD2 and FAE1 protein sequences of Camelina species, e.g., Camelina sativa, mutations in Camelina FAD2 and FAE1 genes, and methods of using the same. In addition, methods of altering Camelina seed composition and/or improving Camelina seed oil quality are disclosed. Furthermore, methods of breeding Camelina cultivars and/or other closely related species to produce plants having altered or improved seed oil and/or meal quality are provided.
Claims29
An isolated polynucleotide encoding a plant fatty acid synthesis polypeptide, wherein the isolated polynucleotide encodes a plant fatty acid desaturase (FAD) polypeptide comprising a sequence sharing at least 93% identity to SEQ ID NO:
- 1, 2, 3, 45, 46, 48, 51, 54, 55, 56, 60, or 61;
- or the isolated polynucleotide encodes a plant fatty acid elongase (FAE) polypeptide comprising a sequence sharing at least 91% identity to SEQ ID NO:
- 4, 5, 6, 47, 49, 50, 52, 53, 57, 58, 59, 62, or 63.
-6. (canceled)
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A plant cell, plant part, plant tissue culture or whole plant comprising at least one FAD2 gene comprising at least one mutation that disrupts and/or alters the function of the at least one FAD2 gene and/or at least one FAE1 gene comprising at least one mutation that disrupts and/or alters the function of the at least one FAE1 gene.
10-15. (canceled)
A method of altering and/or improving fatty acid composition of a Camelina plant cell, plant part, tissue culture or whole plant comprising disrupting and/or altering one, two, or all three copies of one or more fatty acid synthesis genes in said Camelina plant cell, plant part, tissue culture or whole plant.
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A method of breeding Camelina plants to produce altered levels of fatty acids in seed oil and/or meal, wherein the method comprises
- i) making a cross between a Camelina plant with one or more mutations listed in Tables 7-12 with a second Camelina plant to produce a F1 plant;
- ii) backcrossing the F1 plant to the second Camelina plant;
- and
- iii) repeating the backcrossing step to generate a near isogenic line, wherein the one or more mutations are integrated into the genome of the second Camelina plant;
- wherein the near isogenic line derived from the second Camelina plant with the integrated mutations has altered seed oil composition compared to that of the second Camelina plant without the integrated mutations;
- wherein the mutations disrupt and/or alter one, two or three homologous copies of endogenous FAD2 and/or FAE1 genes.
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29-30. (canceled)
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35-36. (canceled)
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Dependencies are inferred from the claim text.