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US 2011/0239322 A1Published application
In vitro flowering/seed set leading to partial or complete male sterility in grasses
AssigneeThe Penn State Research Foundation
InventorsSairam Venkata Rudrabhatla, Rebekah Ellen Templin, Shobha Devi Potlakayala
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Abstract
The present invention relates to a method of manipulating plant development that allows for partial or complete male sterility in grasses.
Claims20
A method for eliciting male sterile or partially male sterile grass plants comprising:
- a) germinating at least one seed on a MS;
- b) transferring the callus to a shoot induction medium followed by a flowering medium comprising at least one substituted phenylurea cytokinin analog;
- and
- c) allowing the callus to develop at least one flower bud.
1 more element
A method for eliciting male sterile or partially male sterile grass plants comprising:
- a) sterilizing grass seeds;
- b) germinating the seeds on basal medium containing an auxin;
- c) removing the end of the shoot, and placing again in basal medium with auxin;
- d) transferring the resultant callus into regeneration medium containing a cytokine, auxin, and CuSO4;
- e) after shoots form from the callus, transferring the callus to the final regeneration medium of basal medium, a substituted phenylurea cytokinin analog, an auxin, a cytokine, and CuSO4.
2 more elements
Dependencies are inferred from the claim text.