Method of diagnosing a respiratory disease

A method of diagnosing a disease state in a subject comprising:

• (a) obtaining nuclear magnetic resonance data on an obtained biological sample from the subject in the form of an x,y-trace;
• (b) performing a statistical analysis on the nuclear magnetic resonance data;
• (c) determining a subject profile for the biological sample based on the statistical analysis;
• (d) comparing the subject profile to a predetermined profile for differentiating between:
• (i) the disease state and a non-diseased state,
• (ii) a first disease state and a second disease state, or
• (iii) the first disease state, the second disease state, and the non-diseased state,
• to provide a diagnosis of the disease state, wherein the comparing does not comprise identification of components of the biological sample.

A method of diagnosing an asthma disease state in a subject comprising:

• (a) measuring the concentration of each of phenylalanine, 2-oxoglutarate, 1-methylnicotinamide, threonine, 0-acetylcarnitine, 1-methylhistamine, succinate, 2-hydroxyisobutyrate, kynurenine, trimethylamine n-oxide, tryptophan, yellow-7.1, homovanillate, adenosine, methylamine, 4-aminohippurate, carnitine, adenine, 3-methyladipate, hippurate, 4-pyridoxate, trigonelline, and tyrosine in an obtained biological sample from the subject;
• (b) performing a statistical analysis on the concentration values obtained in (a);
• (c) determining an asthma state profile for the biological sample based on the statistical analysis;
• (d) comparing the asthma state profile to a predetermined profile for differentiating between chronic stable asthma and a non-diseased state.