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US 2011/0237775 A1Published application
Methods for characterizing glycoproteins and generating antibodies for same
AssigneeInstitute for Systems Biology
InventorsDan MARTIN, Bernd Wollscheid, Rudolf H. Aebersold, Julian Watts
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Abstract
The invention provides methods for preparing a peptide useful in generating an antibody specific for the glycosylated form of a glycopolypeptide wherein the glycosylated portion is isolated and a peptide corresponding to amino acids adjacent to a glycosylated N-linked glycosylation site is prepared.
Claims9
A method to produce an immunizing peptide for generating an antibody specific for the glycosylated form of a glycopolypeptide, comprising:
- (a) isolating a glycosylated portion of said glycopolypeptide;
- (b) identifying by mass spectroscopy an amino acid sequence consisting of 6-40 amino acids whose C-terminus or N-terminus is within 5 amino acids upstream or downstream respectively of the N-terminus or C-terminus respectively of a glycosylated site of said glycosylated portion of glycopolypeptide obtained in step (a), wherein said site has the sequence N—X—S/T;
- (c) producing said immunizing peptide corresponding to the amino acid sequence identified in step (b).
Dependencies are inferred from the claim text.