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AssigneeFukuoka University
InventorsSenji Shirasawa, Takahiro Fujimoto, Toshiyuki Tsunoda, Keiko Doi, Midori Koyanagi
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The inhibitory RNA for inhibiting the expression of ZFAT gene according to this invention is a siRNA comprising a sense RNA having a base sequence of contiguous 20 to 20 bases, preferably 23 to 27 bases, of ZFAT mRNA and an anti-sense RNA having a base sequence complementary to the base sequence of the sense RNA or a shRNA comprising a double-stranded RNA (dsRNA) with the sense RNA connected to the anti-sense RNA via a loop sequence. The inhibitory RNA for inhibiting the expression of ZFAT gene according to this invention decreases a rate of cell proliferation of cancer cells, etc., induces apoptosis of cells including cancer cells, or inhibits an immunoresponse by inhibiting the expression of the ZFAT gene. Therefore, the inhibitory RNA of this invention is useful for development of molecular target agents particularly for cancer cells or immunosuppressive agents.
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An inhibitory RNA for inhibiting the expression of ZFAT gene comprising a double-stranded RNA including a sense RNA having a base sequence of ZFAT mRNA having contiguous 20 to 30 bases of ZFAT mRNA and an anti-sense RNA having a base sequence complementary to the base sequence of the sense RNA.
A cell proliferation inhibiting method wherein an inhibitory RNA for inhibiting the expression of ZFAT gene comprising a sense RNA having a base sequence with contiguous 20 to 30 bases of ZFAT mRNA and an anti-sense RNA having a base sequence complementary to the base sequence of the sense RNA or an expression vector for expressing the expression of the inhibitory RNA for inhibiting the expression of ZFAT gene is introduced into a cell and inhibits proliferation of the cell.
An apoptosis induction method wherein an inhibitory RNA for inhibiting the expression of ZFAT gene comprising a sense RNA having a base sequence with contiguous 20 to 30 bases of ZFAT mRNA and an anti-sense RNA having a base sequence complementary to the base sequence of the sense RNA or an expression vector for expressing the expression of the inhibitory RNA for inhibiting the expression of ZFAT gene is introduced into a cell and inducing apoptosis of the cell or inhibits immune response of the cell in a living body.
An immune inhibition method wherein an inhibitory RNA for inhibiting the expression of ZFAT gene comprising a sense RNA having a base sequence with contiguous 20 to 30 bases of ZFAT mRNA and an anti-sense RNA having a base sequence complementary to the base sequence of the sense RNA or an expression vector for expressing the expression of the inhibitory RNA for inhibiting the expression of ZFAT gene is introduced into a cell and inhibiting an immune response in a living body.
A medical composition wherein an inhibitory RNA for inhibiting the expression of ZFAT gene comprising a double-stranded RNA composed of a sense RNA having a base sequence with contiguous 20 to 30 bases of ZFAT mRNA and an anti-sense RNA having a base sequence complementary to the base sequence of the sense RNA or an expression vector for expressing the inhibitory RNA for inhibiting the expression of ZFAT gene is contained as an active ingredient capable of treating or preventing a diseases including without limitation cancer by inhibiting cell proliferation or inducing apoptosis or inhibiting an immune response.
Dependencies are inferred from the claim text.