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OwnerMark Aaron Emalfarb, Irina Vladimirovna Solovjeva +6inventors
InventorsMark Aaron Emalfarb, Irina Vladimirovna Solovjeva, Arie Ben-Bassat, Richard P. Burlingame, Vladimir Mikhaylovich Chernoglazov, Oleg Nicolaevich Ocounev, Philip T. Olson, Arkady Panteleimonovich Sinitsyn
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The subject invention relates to novel compositions of neutral and/or alkaline cellulase and methods for obtaining neutral and/or alkaline cellulase compositions from Chrysosporium cultures, in particular Chrysosporium lucknowense. This invention also provides mutants and methods of generating mutants of Chrysosporium capable of producing neutral and/or alkaline cellulase. This invention also relates to the genes encoding the enzymes comprising the neutral and/or alkaline cellulase composition. In addition, this invention provides methods of culturing Chrysosporium to produce neutral and/or alkaline cellulases. The neutral and/or alkaline cellulase compositions of the subject invention can be used in a variety of processes including stone washing of clothing, detergent processes, deinking, color brightening, depilling and biobleaching of paper and pulp and treatment of waste streams. The present invention also relates to the isolation and purification of cellulase enzymes, having glucanase and cellobiohydrolase activity, and useful for stonewashing applications.
A composition having neutral and/or alkaline cellulase activity, obtained by a method which comprises growing a wild type or mutant fungus of the genus Chrysosporium in culture in a suitable medium, wherein the fungus is Chrysosporium lucknowense, Chrysosporium pannorum, Chrysosporium keratinophilum, Chrysosporium lobatum, Chrysosporium merdarium, Chrysosporium queenslandicum, or Chrysosporium tropicum.
A composition for the enzymatic treatment of cellulosic fibers or cellulosic fabrics, comprising a cellulase whose amino acid sequence is encoded by a nucleic acid sequence from a wild-type or mutant fungus of the genus Chrysosporium, said composition having a pH between about 8.0 and about 12.0, wherein the fungus is selected from the group consisting of Chrysosporium lucknowense, Chrysosporium pannorum, Chrysosporium pruinosum, Chrysosporium keratinophilum, Chrysosporium lobatum, Chrysosporium merdarium, Chrysosporium queenslandicum, and Chrysosporium tropicum.
Dependencies are inferred from the claim text.