Using phage epitopes to profile the immune response
AssigneesThe Regents of the University of Michigan, Armune Biosciences, Inc.
InventorsArul M. Chinnaiyan, Xiaoju Wang, Alex Tsodikov, Jeanne Ohrnberger
Life of this patent
Estimating the term…
Abstract
The present disclosure provides compositions and methods for using one or more polypeptide probes to profile an immune response. The polypeptide probe can be used to detect one or more antibodies from a sample. Furthermore, the present disclosure provides methods and compositions for characterizing a cancer based on the detection of one or more antibodies, such as autoantibodies.
Claims32
An antibody profiling panel comprising:
- a plurality of polypeptide probes, wherein at least one of said polypeptide probes comprises:
- a full-length or fragment of a protein listed in Table 1 or polypeptide sequence selected from SEQ ID NO:
- 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, or 141,
- wherein each of said probes in said plurality of polypeptide probes is capable of being specifically bound by an antibody.
A method for screening a subject for a cancer comprising:
- detecting in a sample obtained from a subject an expression level of one or more antibodies with at least one polypeptide probe comprising:
- a full-length or fragment of a protein listed in Table 1 or
- a polypeptide sequence selected from SEQ ID NO:
- 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, or 141;
- wherein said expression level is indicative of the presence, absence, or stage of said cancer.
A method of recommending a biopsy be obtained comprising:
- (a) contacting a biological sample obtained from a subject with one or more probes for an antibody, wherein said subject has a PSA level greater than about 2.5 ng/mL;
- (b) detecting an expression level of an antibody;
- and
- (c) recommending a biopsy be obtained based on said expression level of said antibody.
A method of screening a subject for a cancer comprising:
- (a) contacting a biological sample obtained from said subject with one or more probes for an antibody, wherein said subject has a positive biopsy result for cancer;
- and
- (b) detecting an expression level for said antibody, wherein said expression level is indicative of the presence, absence, or stage of said cancer.
Dependencies are inferred from the claim text.