Multiplexed Assay Using Encoded Solid Support Matrices
AssigneeNexus Biosystems, Inc.
InventorsMichael P. Nova, Andrew E. Senyei, Zahra Parandoosh, Gary S. David, Xiao-Yi Xiao
Life of this patent
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Abstract
In a multiplexed assay, each molecule of a plurality of molecules is attached to a support matrix with a substrate adapted for attachment and/or synthesis of molecules and an integrally-formed memory device with an optically-encoded identifier to uniquely identify the molecule attached to the substrate. The molecules are exposed to one or more processing conditions then placed within the path of an optical detector adapted to read the optically-encoded identifier and measure biochemical processes on each support matrix. The support matrices may be singulated to be read by the optical detector one at a time.
Claims20
A multiplexed assay, comprising:
- attaching each molecule of a plurality of molecules to one of a plurality of separate support matrices, each support matrix comprising a substrate adapted for attachment of a molecule and an integrally-formed pre-encoded memory device comprising an optically-encoded identifier to uniquely identify the molecule attached to the substrate;
- exposing the plurality of molecules to one or more processing conditions in suspension;
- placing the plurality of support matrices into a vessel having a constricted section feeding into an optically-transparent tube disposed within the path of an optical detector, the optical detector adapted to read the optically-encoded identifier as the support matrix passes through the optically-transparent tube;
- and
- measuring biochemical processes on each support matrix.
An assay for evaluating a plurality of molecules, comprising:
- linking each of the molecules to a separate support matrix having an integral optically-readable memory pre-encoded with a unique identity, wherein the unique identity is associated with the molecule linked to the support matrix;
- exposing each molecule and its linked support matrix to one or more reagents in suspension, wherein at least one of the one or more reagents is conjugated with a label;
- placing the plurality of support matrices into a vessel having a constricted section feeding into an optically-transparent tube disposed within the path of an optical detector, the optical detector adapted to read the optically-readable memory as the support matrix passes through the optically-transparent tube;
- and
- directing the support matrices past an analytical instrument adapted to detect the label for measuring biochemical processes on each support matrix.
A multiplexed assay, comprising:
- linking each molecule of a plurality of molecules to a separate support matrix comprising a bead or particle having a unique optically-encoded identifier embedded or encased within the support matrix, wherein the optically-encoded identifier is associated with the molecule linked to the support matrix;
- exposing the plurality of molecules and their corresponding support matrices to one or more processing steps in suspension to produce a plurality of processed molecules, wherein at least one of the one or more processing steps comprises attaching a detectable label for measuring biochemical activity within each processed molecule;
- and
- placing the plurality of support matrices into a vessel having a constricted section feeding into an optically-transparent tube disposed within the path of an optical detector adapted to read the optically-encoded identifier and a detection path of an analytical instrument adapted to detect the detectable label for measuring biochemical activity on each support matrix, wherein the measured biochemical activity is associated with the optically-encoded identifier for the corresponding support matrix and, hence, with the processed molecule that is associated with the optically-encoded identifier.
Dependencies are inferred from the claim text.