Methods of mapping genomic methylation patterns
AssigneeLife Technologies Corporation
InventorsKevin Clancy, Gavin Meredith, Christopher Adams, Daniel Krissinger
Life of this patent
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Abstract
The invention relates to sample analysis work flows for increasing the efficiency of experiments. Compositions and methods are described for selectively increase the abundance of methylated nucleic acid over non-methylated nucleic acid, followed by analysis of the nucleic acid to identify methylation sites.
Claims24
A method of mapping methylated cytidine in a genome of an organism comprising,
- (a) isolating methylated DNA fragments from the organism,
- (b) sequencing a first portion of the methylated DNA fragments isolated from the genome of the organism thereby producing a first DNA sequence,
- (c) sequencing a second portion of the methylated DNA isolated from the genome of the organism which has been treated such that non-methylated cytidine is converted to uridine thereby producing a second DNA sequence, and
- (d) aligning the second DNA sequence with the first DNA sequence thereby producing a map of methylated cytidine in the genome of the organism.
A method of mapping methylated cytidine in a genome of an organism comprising:
- (a) isolating from the genome of the organism, methylated DNA fragments,
- (b) splitting the isolated methylated DNA fragments into at least a first portion and a second portion,
- (c) treating the first portion of isolated methylated DNA fragments such that non-methylated cytidine is converted to uridine,
- (d) sequencing the first and second portions of isolated methylated DNA, and
- (e) mapping the sequence of the first portion of the isolated methylated DNA to the sequence of the second portion of the isolated methylated DNA.
A kit for mapping methylated cytidine in a genome of an organism comprising a methylated DNA binding substance bound to a solid support.
Dependencies are inferred from the claim text.