Amelogenin SNP on Chromosome X
AssigneeLife Technologies Corporation
InventorsJulio Mulero, Robert Green, Robert Lagace, Lori Hennessy
Life of this patent
Estimating the term…
Abstract
Disclosed are methods for gender determination in the intron 1 region of the amelogenin locus and a newly discovered single nucleotide polymorphism (SNP) within the X chromosome of the amelogenin locus which can cause allelic dropout. Also disclosed are kits useful in gender determination.
Claims51
A method for gender determination comprising
- a. binding a first primer to a first Amelogenin primer binding site in a target nucleic acid sequence;
- b. binding a second primer to a second Amelogenin primer binding site in said target nucleic acid sequence;
- wherein said second primer binds to a single nucleotide polymorphism (SNP) on chromosome X in said target sequence;
- c. amplifying said target nucleic acid, wherein the amplifying yields at least a first amplified sequence;
- and
- d. detecting the at least said first amplified sequence.
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A method for gender determination comprising:
- a. annealing at least a first primer to at least a first primer binding site in a target nucleic acid sequence;
- b. annealing at least a second primer to at least a second primer binding site;
- c. optionally, annealing a third primer specific to a SNP on chromosome X;
- d. amplifying said target nucleic acid, wherein the amplifying yields at least a first amplified sequence;
- and
- e. detecting at least a first amplified sequence.
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A method comprising:
- a. co-amplifying a plurality of STR loci and the Amelogenin locus in a multiplex amplification reaction, wherein the product of the reaction is a mixture of a plurality of amplified alleles from each of the co-amplified loci;
- and
- b. evaluating the plurality of amplified alleles in the mixture to determine the alleles present at each of the loci analyzed.
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A kit for gender determination comprising:
- a first primer with a 3′ terminus nucleotide capable of hybridizing to position 83 within intron 1 of Amelogenin chromosome X allele as shown in SEQ ID NO:1 or its complement, a second primer with a 3′ terminus nucleotide capable of hybridizing to position 83 within intron 1 of the Amelogenin chromosome X allele as shown in SEQ ID NO:2 or its complement, and a third primer which hybridizes upstream of position 83 within intron 1 of Amelogenin chromosome X allele as shown in SEQ ID NO:1 and SEQ ID NO:2 and SEQ ID NO:3;
- wherein said primers are used in a PCR reaction and
- wherein amplification products from both SEQ ID NO:1 and SEQ ID NO:3 or SEQ ID NO:2 and SEQ ID NO:3 determines the gender as male and an amplification product from only SEQ ID NO:1 or SEQ ID NO:2 determines the gender as female.
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Dependencies are inferred from the claim text.