Aptamer-Based Colorimetric Sensor Systems
AssigneeThe Board of Trustees of the University of Illinois
InventorsYi Lu, Juewen Liu
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Abstract
The present invention provides an aptamer-based colorimetric sensor system for determining the presence and optionally the concentration of an analyte in a sample. Methods of utilizing the sensor system and kits that include the sensor also are provided. The sensor utilizes a linker and oligonucleotide functionalized particles to form an aggregate, which disaggregates in response to the analyte.
Claims23
A sensor system for detecting an analyte, comprising:
- an aggregate comprising:
- a linker comprising an aptamer that is capable of folding in response to the analyte;
- and
- first particles coupled to a first oligonucleotide, the first oligonucleotide complementary to at least a portion of the aptamer.
2-18. (canceled)
A method of detecting an analyte, comprising:
- combining an aggregate with a sample;
- and
- detecting a color change responsive to the analyte, the aggregate comprising:
- a linker comprising an aptamer that folds in response to the analyte;
- and
- first particles coupled to a first oligonucleotide, the first oligonucleotide complementary to at least a portion of the aptamer.
20-36. (canceled)
A kit for the detection of an analyte, comprising:
- a system for forming aggregates, comprising:
- a linker comprising an aptamer that folds in response to the analyte;
- and
- first particles coupled to a first oligonucleotide, the first oligonucleotide complementary to at least a portion of the aptamer;
- and
- at least one first container containing the aggregate forming system, where a sample may be added to a container selected from the group comprising the first container and a second container.
38-49. (canceled)
A method for determining the sensitivity and selectivity of an aptamer to an analyte, comprising:
- combining an aggregate with the analyte;
- detecting a color change responsive to the analyte, the aggregate comprising:
- a linker comprising a DNA strand;
- and
- first particles coupled to a first oligonucleotide, the first oligonucleotide complementary to at least a portion of the DNA strand;
- and
- determining if the DNA strand folded to provide the color change.
Dependencies are inferred from the claim text.