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US 2011/0236978 A1Published application
Reprogramming cells toward a pluripotent state
AssigneeFraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.
InventorsAlexandra Stolzing, Antje Arnold, Jeremy Brown
Life of this patent
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Abstract
The present invention relates to a process for preparing reprogrammed cells, in particular for preparing pluripotent and multipotent stem cells and to the pluripotent and multipotent stem cells prepared by said processes.
Claims15
A process for preparing reprogrammed cells in vitro, comprising:
- a) providing cells to be reprogrammed;
- b) introducing reprogramming mRNA molecules into the cells provided in step a), wherein the reprogramming mRNA molecules are encoding at least one reprogramming protein selected from the group consisting of Ronin, Oct4, Klf4, Sox2, Nanog and TERT;
- c) culturing the cells obtained in step b) in a cell culture medium and under a condition suitable to allow translation of the transfected reprogramming mRNA molecules so as to obtain reprogrammed cells.
A process for reprogramming recipient cells of a mammal in vivo comprising:
- introducing reprogramming mRNA molecules into the recipient cells of the mammal, wherein the reprogramming mRNA molecules are encoding at least one reprogramming protein selected form the group consisting of Ronin, Oct4, Klf4, Sox2, Nanog and TERT.
Dependencies are inferred from the claim text.