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US 2011/0236971 A2Published application
Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
OwnerMaksym Vodyanyk, Igor Slukvininventors
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Abstract
Methods for obtaining multipotent Apelin receptor-positive lateral plate mesoderm cells, mesenchymal stem cells, and mesangioblasts under serum-free conditions are disclosed
Claims29
A method of generating a clonal population of primate mesenchymal stem cells, the method comprising the steps of:
- culturing a heterogeneous, single-cell suspension of primate cells that contains mesenchymal progenitors in a serum-free, semi-solid medium containing between about 5 and about 100 ng/ml bFGF until independent colonies form;
- and
- culturing one of the independent colonies in a serum-free, liquid medium containing between about 5 and about 100 ng/ml bFGF to obtain a substantially pure clonal population of MSCs.
A cell population comprising:
- a substantially pure line of clonally-derived mesenchymal stem cells positive at least for CD44, CD56, CD73, CD146, CD140a and CD105, but negative for CD31, CD43, CD45 and VE cadherin.
A method of generating a population of primate Apelin receptor-positive lateral plate mesoderm cells, the method comprising the steps of;
- culturing primate pluripotent stem cells in a medium that supports differentiation until Apelin receptor is expressed
- isolating Apelin receptor-positive lateral plate mesoderm cells.
Dependencies are inferred from the claim text.