MULTICISTRONIC siRNA CONSTRUCTS TO INHIBIT TUMORS
AssigneeThe Board of Trustees of the University of Illinois
InventorsJasti S. Rao, Christopher S. Gondi, Sajani S. Lakka
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Abstract
Multicistronic short interfering RNA constructs targeting in various combinations a human urokinase-type plasminogen activator receptor (uPAR), human urokinase-type plasminogen activator (uPA), human matrix metalloprotease 9 (MMP-9) and cathepsin B (CB) inhibit tumors.
Claims23
A multicistronic short interfering RNA construct comprising at least a first and a second self-complementary sequence, wherein the construct is used to inhibit tumor formation and to regress pre-formed tumors.
A short interfering RNA molecule selected from the group consisting of RNA molecules targeted to:
- (a) urokinase-type plasminogen activator receptor (uPAR) and matrix metalloprotease 9 (MMP-9) the short interfering RNA molecule, comprising the nucleic acid sequence CUACAGCAGUGGAGAGCGAUU-loop-AAUCGCUCUCCACUGCUGUAG-intervening sequence —CAAGUGGCACCACCACAACAA-loop-UUGUUGUGGUGGUGCCACUUG (SEQ ID NOS 9 & 10, respectively);
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- (b) urokinase-type plasminogen activator receptor (uPAR) and urokinase-type plasminogen activator (uPA), the short interfering RNA molecule comprising the nucleic acid sequence UGAGAGCCCUGCUGGCGCGCC-loop-GGCGCGCCAGCAGGGCUCUCA-intervening sequence —CUACAGCAGUGGAGAGCGAUU-loop-AAUCGCUCUCCACUGCUGUAG (SEQ ID NOS 11 & 9, respectively).
Dependencies are inferred from the claim text.